Dr. Erdinc Sezgin
Eggeling Lab, Weatherall Institute of Molecular Medicine
John Raddcliffe Hospital, University of Oxford
Oxford
OX39DS
United Kingdom
email: erdinc.sezgin@rdm.ox.ac.uk
twitter: @Sciezgin
Eggeling Lab, Weatherall Institute of Molecular Medicine
John Raddcliffe Hospital, University of Oxford
Oxford
OX39DS
United Kingdom
email: erdinc.sezgin@rdm.ox.ac.uk
twitter: @Sciezgin
Research Interest
My research projects aim at a thorough understanding of the role of heterogeneity in molecular membrane organization on the functionality of the membrane-related immunological signalling events.
Objectives:
1. To investigate how proteins involved in T-cell signalling partition in the heterogeneous membrane: into the ordered or disordered membrane domains?
2. Is the partitioning behaviour of a protein essential for its functionality (or is functionality preserved in the respective other phase)?
3. How is membrane heterogeneity modulated in T-cells and antigen-presenting cells?
4. Can a model membrane system be instituted as an in-vitro platform for investigating membrane-related aspects of the T-cell activation process?
My research projects aim at a thorough understanding of the role of heterogeneity in molecular membrane organization on the functionality of the membrane-related immunological signalling events.
Objectives:
1. To investigate how proteins involved in T-cell signalling partition in the heterogeneous membrane: into the ordered or disordered membrane domains?
2. Is the partitioning behaviour of a protein essential for its functionality (or is functionality preserved in the respective other phase)?
3. How is membrane heterogeneity modulated in T-cells and antigen-presenting cells?
4. Can a model membrane system be instituted as an in-vitro platform for investigating membrane-related aspects of the T-cell activation process?
CV
- BSc (2004-2009), Yeditepe University, Department of genetics and Bioengineering, Istanbul, Turkey
- PhD (2010-2012), Petra Schwille Lab, Biotechnology Center of Technical University Dresden, Dresden, Germany
- Postdoc (2013), Kai Simons Lab, Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
- EMBO Postdoctoral Fellow (2014), Christian Eggeling Lab, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
- Marie Skłodowska-Curie Postdoctoral Fellow (2015-2017), Christian Eggeling Lab, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
- BSc (2004-2009), Yeditepe University, Department of genetics and Bioengineering, Istanbul, Turkey
- PhD (2010-2012), Petra Schwille Lab, Biotechnology Center of Technical University Dresden, Dresden, Germany
- Postdoc (2013), Kai Simons Lab, Max Planck Institute of Cell Biology and Genetics, Dresden, Germany.
- EMBO Postdoctoral Fellow (2014), Christian Eggeling Lab, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
- Marie Skłodowska-Curie Postdoctoral Fellow (2015-2017), Christian Eggeling Lab, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, UK.
Publications
Please visit Erdinc Sezgin's Google Scholar profile for details of the listed publications.
Please visit Erdinc Sezgin's Google Scholar profile for details of the listed publications.
2018
40. Xiong Y, Jentzsch AV, Osterrieth JWM, Sezgin E, Sazanovich IV, Reglinski K, Galiani S, Parker AW, Eggeling C and
Anderson HL. Spironaphthoxazine switchable dyes for biological imaging Chemical Science, (2018)
39. Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J. Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling. European Journal of Immunology, (2018)
2017
38. Plochberger B, Röhrl C, Preiner J, Rankl C, Brameshuber M, Madl J, Bittman R, Ros R, Sezgin E, Eggeling C, Hinterdorfer P, Stangl H, Schütz GJ. HDL particles incorporate into lipid bilayers - a combined AFM and single molecule fluorescence microscopy study. Scientific Reports, 7(1), 15886, (2017)
37. Sezgin E, Schneider F, Zilles V, Urbančič I, Garcia E, Waithe D, Klymchenko AS, Eggeling C. Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy. Biophysical Journal, (2017)
36. Diepold A, Sezgin E, Huseyin M, Mortimer T, Eggeling C, Armitage JP. A dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome. Nature Communications, 8, 15940, (2017)
35. Sezgin E, Azbazdar Y, Ng XW, Teh C, Simons K, Weidinger G, Wohland T, Eggeling C, Ozhan G. Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments. FEBS Journal, (2017)
34. Sezgin E. Super-resolution optical microscopy for studying membrane structure and dynamics. Journal of Physics Condensed Matter, (2017)
33. Aron M, Browning R, Carugo D, Sezgin E, Bernardino de la Serna J, Eggeling C, Stride E. Spectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order. BMC Bioinformatics, 18 (1), 254 (2017)
32. Rissanen S, Grzybek M, Orlowski A, Rog T, Cramariuc O, Levental I, Eggeling C, Sezgin E*, Vattulainen I*. Phase Partitioning of GM1 and Its Bodipy-Labeled Analog Determine Their Different Binding to Cholera Toxin Frontiers in Physiology, (2017) (*co-correspondence)
31. Schneider F, Waithe D, Clausen MP, Galiani S, Koller T, Ozhan G, Eggeling C*, Sezgin E*. Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS. Molecular Biology of the Cell, (2017) (*co-correspondence)
30. Sezgin E, Levental I, Mayor S, Eggeling C. The mystery of membrane organization: composition, regulation and roles of lipid rafts Nature Reviews Molecular Cell Biology, (2017)
29. Pereno V, Carugo D, Bau L, Sezgin E, de la Serna JB, Eggeling E, Stride E. Electroformation of giant unilamellar vesicles on stainless steel electrodes, ACS Omega, 2(3), 994–1002 (2017)
28. Koller T, Blok S, Santos AM, Oszmiana A, Davis DM, Sezgin E*, Eggeling C. Rituximab capping triggers intracellular reorganization of B cells. Matters, (2017) (*correspondence)
27. Amaro M, Reina F, Hof M, Eggeling C, Sezgin E*. Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane. Journal of Physics D: Applied Physics, 50(13), 134004-134013, (2017)
26. Fritzsche M, Li D, Colin-York H, Chang VT, Moeendarbary E, Felce JH, Sezgin E, Charras G, Betzig B, Eggeling C. Self-organizing actin patterns shape membrane architecture but not cell mechanics. Nature Communications, 8, 14347 (2017)
25. Carugo D, Aron M, Sezgin E, Bernardino de la Serna J, Kuimova MK, Eggeling C, Stride E. Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles. Biomaterials, 113, 105-117 (2016)
2016
24. Xiong Y, Rivera-Fuentes P, Sezgin E, Vargas Jentzsch A, Eggeling C, Anderson HL. Photoswitchable Spiropyran Dyads for Biological Imaging. Organic Letters, 18(15), 3666-3669 (2016)
23. Steshenko O, Andrade DM, Honigmann A, Mueller V, Schneider F, Sezgin E, Hell SW, Simons M, Eggeling C. Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy. Biophysical Journal, 110(11), 2441-2450 (2016)
22. Hölttä-Vuori M, Sezgin E, Eggeling C, Ikonen E. Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation. Traffic, (2016)
21. Sezgin E, Eggeling C. Chasing Lipid Rafts. Pan European Networks: Science & Technology, 18, 162-163, (2016)
20. Sezgin E*, Can FB, Schneider F, Clausen MP, Galiani S, Stanly TA, Waithe D, Colaco A, Honigmann A, Wuestner D, Platt F, Eggeling C*. A comparative study on fluorescent cholesterol analogs as versatile cellular reporters. Journal of Lipid Research, (2016). (*co-correspondence)
2015
19. Waithe D, Clausen MP, Sezgin E, Eggeling C. FoCuS-point: Software for STED Fluorescence Correlation and Time-Gated Single Photon Counting. Bioinformatics, (2015).
18. Vicidomini G, Ta H, Honigmann A, Mueller V, Clausen MP, Waithe D, Galiani S, Sezgin E, Diaspro A, Hell SW, Eggeling C. STED-FLCS: An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics. Nano Letters, 15(9), 5912-5918, (2015).
17. Clausen MP, Sezgin E, de la Serna JB, Waithe D, Christoffer Lagerholm B, Eggeling C. A straightforward approach for gated STED-FCS to investigate lipid membrane dynamics. Methods, 88, 67-75, (2015).
16. Sezgin E, Gutmann T, Buhl T, Dirkx R, Grzybek M, Coskun U, Solimena M, Simons K, Levental I*, Schwille P*. Adaptive lipid packing and bioactivity in the membrane domains. PLOS ONE, 10(4); e0123930 (2015).
15. Sezgin E, Davis SJ, Eggeling C. Membrane nanoclusters—tails of the unexpected. Cell, 161, 433-434 (2015).
14. Sezgin E*, Waithe D, Bernardino de la Serna J, Eggeling C*. Spectral Imaging to measure heterogeneity in membrane lipid packing. Chemphyschem, 16(7), 1387-1394. (2015). (*co-correspondence)
13. Beck-García K, Beck-García E, Bohler S, Zorzin C, Sezgin E, Levental I, Alarcón B, Schamel WW. Nanoclusters of the resting T cell antigen receptor (TCR) localize to non-raft domains. BBA Molecular Cell Research, 1853 (4), 802-809, (2015).
2014
12. Honigmann A, Mueller V, Ta H, Schoenle A, Sezgin E, Hell SW, Eggeling C. (2014) Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells. Nature Communications, 5, 5412, (2014).
11. Sezgin E*, Sadowski T, Simons K*. Measuring Lipid Packing of Model and Cellular Membranes with Environment Sensitive Probes. Langmuir, 30, 8160-8166, (2014). (*co-correspondence)
2013
10. Zhou Y, Maxwell KN, Sezgin E, Lu M, Liang H, Hancock JF, Dial EJ, Lichtenberger LM, Levental I. Bile acids modulate signaling by functional perturbation of plasma membrane domains. Journal of Biological Chemistry, 288, 35660-35670, (2013).
9. Sezgin E, Chwastek G, Aydogan G, Levental I, Simons K, Schwille P. Photoconversion of Bodipy-Labeled Lipid Analogues. Chembiochem, 14, 695-698, (2013).
8. Ozhan G, Sezgin E, Wehner D, Pfister A, Kuhl S, Kagermeier-Schenk B, Kuhl M, Schwille P, Weidinger G. Lypd6 enhances Wnt/beta-catenin signaling by promoting Lrp6 phosphorylation in raft plasma membrane domains. Developmental Cell, 26, 331-345, (2013).
2012
7. Sezgin E, Kaiser H-J, Baumgart T, Schwille P, Simons K, Levental I. Elucidating membrane structure and protein behavior using giant plasma membrane vesicles. Nature Protocols, 7, 1042-1051, (2012).
6. Sezgin E, Levental I, Grzybek M, Schwarzmann G, Mueller V, Honigmann A, Belov VN, Eggeling C, Coskun U, Simons K, Schwille P. Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes. BBA-Biomembranes, 1818, 1777-1784, (2012).
5. Dubavik A*, Sezgin E*, Lesnyak V, Gaponik N, Schwille P, Eychmueller A. Penetration of Amphiphilic Quantum Dots through Model and Cellular Plasma Membranes. ACS Nano, 6, 2150-2156, (2012). (*co-first author)
4. Saenz JP, Sezgin E, Schwille P, Simons K. Functional convergence of hopanoids and sterols in membrane ordering. PNAS, 109, 14236-14240, (2012).
3. Sezgin E and Schwille P. Model membrane platforms to study protein-membrane interactions. Molecular Membrane Biology, 29, 144-154,(2012).
2011
2. Sezgin E and Schwille P. Fluorescence Techniques to Study Lipid Dynamics. Cold Spring Harbor Perspectives in Biology, 3, (2011).
2009
1. Karatas OF, Sezgin E, Aydin O, Culha M. Interaction of gold nanoparticles with mitochondria. Colloids and Surfaces B-Biointerfaces, 71, 315-318, (2009).
40. Xiong Y, Jentzsch AV, Osterrieth JWM, Sezgin E, Sazanovich IV, Reglinski K, Galiani S, Parker AW, Eggeling C and
Anderson HL. Spironaphthoxazine switchable dyes for biological imaging Chemical Science, (2018)
39. Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J. Infection with a Brazilian isolate of Zika virus generates RIG-I stimulatory RNA and the viral NS5 protein blocks type I IFN induction and signaling. European Journal of Immunology, (2018)
2017
38. Plochberger B, Röhrl C, Preiner J, Rankl C, Brameshuber M, Madl J, Bittman R, Ros R, Sezgin E, Eggeling C, Hinterdorfer P, Stangl H, Schütz GJ. HDL particles incorporate into lipid bilayers - a combined AFM and single molecule fluorescence microscopy study. Scientific Reports, 7(1), 15886, (2017)
37. Sezgin E, Schneider F, Zilles V, Urbančič I, Garcia E, Waithe D, Klymchenko AS, Eggeling C. Polarity-Sensitive Probes for Superresolution Stimulated Emission Depletion Microscopy. Biophysical Journal, (2017)
36. Diepold A, Sezgin E, Huseyin M, Mortimer T, Eggeling C, Armitage JP. A dynamic and adaptive network of cytosolic interactions governs protein export by the T3SS injectisome. Nature Communications, 8, 15940, (2017)
35. Sezgin E, Azbazdar Y, Ng XW, Teh C, Simons K, Weidinger G, Wohland T, Eggeling C, Ozhan G. Binding of canonical Wnt ligands to their receptor complexes occurs in ordered plasma membrane environments. FEBS Journal, (2017)
34. Sezgin E. Super-resolution optical microscopy for studying membrane structure and dynamics. Journal of Physics Condensed Matter, (2017)
33. Aron M, Browning R, Carugo D, Sezgin E, Bernardino de la Serna J, Eggeling C, Stride E. Spectral imaging toolbox: segmentation, hyperstack reconstruction, and batch processing of spectral images for the determination of cell and model membrane lipid order. BMC Bioinformatics, 18 (1), 254 (2017)
32. Rissanen S, Grzybek M, Orlowski A, Rog T, Cramariuc O, Levental I, Eggeling C, Sezgin E*, Vattulainen I*. Phase Partitioning of GM1 and Its Bodipy-Labeled Analog Determine Their Different Binding to Cholera Toxin Frontiers in Physiology, (2017) (*co-correspondence)
31. Schneider F, Waithe D, Clausen MP, Galiani S, Koller T, Ozhan G, Eggeling C*, Sezgin E*. Diffusion of lipids and GPI-anchored proteins in actin-free plasma membrane vesicles measured by STED-FCS. Molecular Biology of the Cell, (2017) (*co-correspondence)
30. Sezgin E, Levental I, Mayor S, Eggeling C. The mystery of membrane organization: composition, regulation and roles of lipid rafts Nature Reviews Molecular Cell Biology, (2017)
29. Pereno V, Carugo D, Bau L, Sezgin E, de la Serna JB, Eggeling E, Stride E. Electroformation of giant unilamellar vesicles on stainless steel electrodes, ACS Omega, 2(3), 994–1002 (2017)
28. Koller T, Blok S, Santos AM, Oszmiana A, Davis DM, Sezgin E*, Eggeling C. Rituximab capping triggers intracellular reorganization of B cells. Matters, (2017) (*correspondence)
27. Amaro M, Reina F, Hof M, Eggeling C, Sezgin E*. Laurdan and Di-4-ANEPPDHQ probe different properties of the membrane. Journal of Physics D: Applied Physics, 50(13), 134004-134013, (2017)
26. Fritzsche M, Li D, Colin-York H, Chang VT, Moeendarbary E, Felce JH, Sezgin E, Charras G, Betzig B, Eggeling C. Self-organizing actin patterns shape membrane architecture but not cell mechanics. Nature Communications, 8, 14347 (2017)
25. Carugo D, Aron M, Sezgin E, Bernardino de la Serna J, Kuimova MK, Eggeling C, Stride E. Modulation of the molecular arrangement in artificial and biological membranes by phospholipid-shelled microbubbles. Biomaterials, 113, 105-117 (2016)
2016
24. Xiong Y, Rivera-Fuentes P, Sezgin E, Vargas Jentzsch A, Eggeling C, Anderson HL. Photoswitchable Spiropyran Dyads for Biological Imaging. Organic Letters, 18(15), 3666-3669 (2016)
23. Steshenko O, Andrade DM, Honigmann A, Mueller V, Schneider F, Sezgin E, Hell SW, Simons M, Eggeling C. Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy. Biophysical Journal, 110(11), 2441-2450 (2016)
22. Hölttä-Vuori M, Sezgin E, Eggeling C, Ikonen E. Use of BODIPY-cholesterol (TF-Chol) for visualizing lysosomal cholesterol accumulation. Traffic, (2016)
21. Sezgin E, Eggeling C. Chasing Lipid Rafts. Pan European Networks: Science & Technology, 18, 162-163, (2016)
20. Sezgin E*, Can FB, Schneider F, Clausen MP, Galiani S, Stanly TA, Waithe D, Colaco A, Honigmann A, Wuestner D, Platt F, Eggeling C*. A comparative study on fluorescent cholesterol analogs as versatile cellular reporters. Journal of Lipid Research, (2016). (*co-correspondence)
2015
19. Waithe D, Clausen MP, Sezgin E, Eggeling C. FoCuS-point: Software for STED Fluorescence Correlation and Time-Gated Single Photon Counting. Bioinformatics, (2015).
18. Vicidomini G, Ta H, Honigmann A, Mueller V, Clausen MP, Waithe D, Galiani S, Sezgin E, Diaspro A, Hell SW, Eggeling C. STED-FLCS: An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics. Nano Letters, 15(9), 5912-5918, (2015).
17. Clausen MP, Sezgin E, de la Serna JB, Waithe D, Christoffer Lagerholm B, Eggeling C. A straightforward approach for gated STED-FCS to investigate lipid membrane dynamics. Methods, 88, 67-75, (2015).
16. Sezgin E, Gutmann T, Buhl T, Dirkx R, Grzybek M, Coskun U, Solimena M, Simons K, Levental I*, Schwille P*. Adaptive lipid packing and bioactivity in the membrane domains. PLOS ONE, 10(4); e0123930 (2015).
15. Sezgin E, Davis SJ, Eggeling C. Membrane nanoclusters—tails of the unexpected. Cell, 161, 433-434 (2015).
14. Sezgin E*, Waithe D, Bernardino de la Serna J, Eggeling C*. Spectral Imaging to measure heterogeneity in membrane lipid packing. Chemphyschem, 16(7), 1387-1394. (2015). (*co-correspondence)
13. Beck-García K, Beck-García E, Bohler S, Zorzin C, Sezgin E, Levental I, Alarcón B, Schamel WW. Nanoclusters of the resting T cell antigen receptor (TCR) localize to non-raft domains. BBA Molecular Cell Research, 1853 (4), 802-809, (2015).
2014
12. Honigmann A, Mueller V, Ta H, Schoenle A, Sezgin E, Hell SW, Eggeling C. (2014) Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells. Nature Communications, 5, 5412, (2014).
11. Sezgin E*, Sadowski T, Simons K*. Measuring Lipid Packing of Model and Cellular Membranes with Environment Sensitive Probes. Langmuir, 30, 8160-8166, (2014). (*co-correspondence)
2013
10. Zhou Y, Maxwell KN, Sezgin E, Lu M, Liang H, Hancock JF, Dial EJ, Lichtenberger LM, Levental I. Bile acids modulate signaling by functional perturbation of plasma membrane domains. Journal of Biological Chemistry, 288, 35660-35670, (2013).
9. Sezgin E, Chwastek G, Aydogan G, Levental I, Simons K, Schwille P. Photoconversion of Bodipy-Labeled Lipid Analogues. Chembiochem, 14, 695-698, (2013).
8. Ozhan G, Sezgin E, Wehner D, Pfister A, Kuhl S, Kagermeier-Schenk B, Kuhl M, Schwille P, Weidinger G. Lypd6 enhances Wnt/beta-catenin signaling by promoting Lrp6 phosphorylation in raft plasma membrane domains. Developmental Cell, 26, 331-345, (2013).
2012
7. Sezgin E, Kaiser H-J, Baumgart T, Schwille P, Simons K, Levental I. Elucidating membrane structure and protein behavior using giant plasma membrane vesicles. Nature Protocols, 7, 1042-1051, (2012).
6. Sezgin E, Levental I, Grzybek M, Schwarzmann G, Mueller V, Honigmann A, Belov VN, Eggeling C, Coskun U, Simons K, Schwille P. Partitioning, diffusion, and ligand binding of raft lipid analogs in model and cellular plasma membranes. BBA-Biomembranes, 1818, 1777-1784, (2012).
5. Dubavik A*, Sezgin E*, Lesnyak V, Gaponik N, Schwille P, Eychmueller A. Penetration of Amphiphilic Quantum Dots through Model and Cellular Plasma Membranes. ACS Nano, 6, 2150-2156, (2012). (*co-first author)
4. Saenz JP, Sezgin E, Schwille P, Simons K. Functional convergence of hopanoids and sterols in membrane ordering. PNAS, 109, 14236-14240, (2012).
3. Sezgin E and Schwille P. Model membrane platforms to study protein-membrane interactions. Molecular Membrane Biology, 29, 144-154,(2012).
2011
2. Sezgin E and Schwille P. Fluorescence Techniques to Study Lipid Dynamics. Cold Spring Harbor Perspectives in Biology, 3, (2011).
2009
1. Karatas OF, Sezgin E, Aydin O, Culha M. Interaction of gold nanoparticles with mitochondria. Colloids and Surfaces B-Biointerfaces, 71, 315-318, (2009).